Preparation and characterization of bioadhesive Bletilla striata polysaccharide and sodium alginate microspheres loaded with Panax notoginseng saponins
10.7501/j.issn.0253-2670.2019.20.009
- VernacularTitle: 生物黏附性三七总皂苷-白及多糖-海藻酸钠复合微球的制备及表征
- Author:
Yu-Jia WU
1
Author Information
1. School of Pharmacy, Nanjing University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Bioadhesive;
Bletilla striata polysaccharide;
Differential scanning calorimetry;
Dispersion;
Ginsenoside Rb1;
Ginsenoside Rd;
Ginsenoside Re;
Ginsenoside Rg1;
Ion cross-linking method;
Microspheres;
Mucoadhesive properties;
Notoginsenoside R1;
Orthogonal design;
Panax notoginseng saponins;
Retention rate;
Sodium alginate;
Sustained-release effect;
Swelling properties
- From:
Chinese Traditional and Herbal Drugs
2019;50(20):4896-4903
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Because of the adhesion of Bletilla striata polysaccharide (BSP), it was mixed with sodium alginate (SA) as a composite carrier to prepare mucoadhesive PNS-BSP composite microspheres. Panax notoginseng saponins (PNS) dispersion with sustained release property was used as an encapsulating drug. Methods: The composite microsphere was prepared by ion cross-linking method. The formulation process was investigated and optimized by single factor test and orthogonal design. The microspheres were evaluated by scanning electron microscope (SEM), particle size distribution, DSC, swelling properties, in vitro mucoadhesive properties, and in vitro release characteristics. Results: PNS-BSP composite microspheres had good roundness, rough surface and wrinkles. The microspheres showed a narrow size distribution. PNS was uniformly dispersed in microspheres in an amorphous state. The microspheres prepared by the best prescription process were stable in process and reproducible. Compared with the microspheres prepared by directly adding PNS, the drug loading, encapsulation efficiency and yield of PNS dispersion microspheres were increased significantly, which were 10.34%, 51.25%, and 82.21%, respectively. The drug loading, encapsulation efficiency, and yield were 4.04%, 12.16%, and 61.35% of PNS microspheres. The addition of BSP increased the swelling properties of the SA microspheres, and significantly increased the retention rate in the stomach of rats. The release of ginsenoside Rg1 in PNS-BSP microspheres was released slower compared to PNS. Conclusion: The bioadhesion of microspheres was increased by the addition of BSP. The drug loading, encapsulation efficiency, and yield of the microspheres were increased by the preparation of PNS as a dispersion, and the microspheres also had a certain sustained-release effect.
