Quality control research of Gesang Jiangtang Capsules based on UPLC fingerprint combined with colorimetry
10.7501/j.issn.0253-2670.2019.21.020
- Author:
Zheng-Cai DU
1
Author Information
1. Guangxi University of Chinese Medicine
- Publication Type:Journal Article
- Keywords:
Fingerprint;
Gesang Jiangtang Capsules;
Ginsenoside Rb1;
Ginsenoside Rg1;
Notoginsenoside R1;
Ombuin;
Ombuoside;
Polysaccharides;
Puerarin;
Quality control;
Rutin;
Similarity evaluation;
Total flavonoids;
Total saponins;
UPLC
- From:
Chinese Traditional and Herbal Drugs
2019;50(21):5266-5272
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the determination and fingerprint of Gesang Jiangtang Capsules (GJC) for its quality control. Methods: UV spectrophotometric method was used for determining polysaccharides, total saponins, and total flavonoids of GJC. Ten batches of GJC were detected and recorded by UPLC. Similarity evaluation was performed by using Similarity Evaluation System for Fingerprint Chromatogram of TCM (2012) to confirm the common peaks. Results: In three batches of GJC, average polysaccharides content, average total saponins content, and average total flavonoids content were 4.56%, 2.97%, and 2.61%, respectively. UPLC fingerprints of 10 batches of GJC were established and 15 common peaks were confirmed. Puerarin, rutin, notoginsenoside R1, ginsenoside Rg1, ombuoside, ginsenoside Rb1, and ombuin were identified by chemical identification of the reference substance, corresponding to peaks 1, 5, 8, 10, 12, 14, and 15. Conclusion: The methods can be used for the quality control of GJC with good precision, accuracy, and reproducibility.
