Establishment of fingerprint of Buyang Huanwu Decoction based on core-shell chromatography and its Q-Orbitrap HRMS/MS analysis
10.7501/j.issn.0253-2670.2019.21.016
- Author:
Dong-Min CAO
1
Author Information
1. School of Traditional Chinese Medicine, Guangdong Pharmaceutical University
- Publication Type:Journal Article
- Keywords:
Amygdalin;
Angelicae Sinensis Radix;
Astragali Radix;
Buyang Huanwu Decoction;
Calycosin;
Carthami Flos;
Chuanxiong Rhizoma;
Core-shell chromatography technology;
Ferulic acid;
Fingerprint;
Formononetin;
Gallic acid;
Guanoside;
Isomucronulatol;
Methyl quinate;
Paeoniae Radix Rubra;
Paeoniflorin;
Persicae Semen;
Pheretima;
Prunasin;
Quality control;
Senkyunolide H;
Succinyl adenosine;
UHPLC-Q-Orbitrap HRMS/MS;
Vanillic acid
- From:
Chinese Traditional and Herbal Drugs
2019;50(21):5239-5245
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish the fingerprint of Buyang Huanwu Decoction (BHD) based on core-shell column for its quality control. Methods: The extract of BHD was separated on an Agilent Poroshell 120 SB-C18 (150 mm × 4.6 mm, 2.7 μm) with a gradient elution. The mobile phase consisted of acetonitrile-0.1% formic acid. The detection wavelength was 280 nm. The similarity evaluation of 15 batches of BHD was carried out by the “Similarity Evaluation System for Chromatographic Fingerprints of TCM”. HPLC-Q-Orbitrap HRMS/MS was used to characterize the 21 chemical compounds of the extract of BHD. Results: The chromatographic fingerprints of 15 batches of BHD were generated 21 common peaks (P1-P21), belonging to all seven medicinal herbs in 38 min. Methyl quinate (P4), calycosin-7-O-β-D-glucoside (P11), calycosin-7-O-β-D-glucoside-6″-O-malonate (P13), ononin (P15), (6αR, 11αR)-9,10-dimethoxypterocarpan-3-O-β-D-glucoside (P16), isomucronulatol-7-O-β-D-glucoside (P17), calycosin (P18), formononetin (P19), (6αR,11αR)-9,10-dimethoxypterocarpan (P20), and isomucronulatol (P21) were from Astragali Radix. Succinyl adenosine (P3), 6-hydroxykaempferol-3,6-diglucoside (P5), and kaempferol-3-O-sophoroside (P10) were from Carthami Flos. Gallic acid (P2) and paeoniflorin (P9) were from Paeoniae Radix Rubra. Vanillic acid (P7) was from Angelicae Sinensis Radix. Guanoside (P1) was from Pheretima. Amygdalin (P6) and prunasin (P8) were from Persicae Semen. Ferulic acid (P12) and senkyunolide H (P14) were from Angelicae Sinensis Radix and Chuanxiong Rhizoma. A total of 21 components were identified by UHPLC-Q-Orbitrap HRMS/MS. It was the first time to establish the UHPLC fingerprint of BHD by core-shell chromatography technology. Conclusion: The method is simple and accurate with a good reproducibility and time consuming, which will provide a basis for the further research on effective constituents in BHD.
