Study on quality control of Miao medicine Inula cappa based on anti-inflammatory active ingredients
10.7501/j.issn.0253-2670.2019.22.027
- Author:
Zhi-Yu ZHANG
1
Author Information
1. Center for Food and Drug Evaluation and Inspection of Guizhou
- Publication Type:Journal Article
- Keywords:
Anti-inflammatory;
Chlorogenic acid;
Cryptochlorogenic acid;
Cynarin;
Fingerprinting;
Inula cappa (Buch.-Ham.ex D.Don) DC.;
Isochlorogenic acid A;
Isochlorogenic acid B;
Isochlorogenic acid C;
Luteolin;
Neochlorogenic acid;
Quantitative control
- From:
Chinese Traditional and Herbal Drugs
2019;50(22):5571-5576
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the quality control method of Miao medicine Inula cappa based on anti-inflammatory active ingredients. Methods: Firstly, a representative and characteristic chemical composition of effective components in I. cappa was established as an indicator component for multi-index quantitative fingerprinting. Then, the quality of I. cappa in different areas of Guizhou Province was evaluated by quantitative analysis of multiple indicators and fingerprint analysis. Results: The HPLC fingerprints and multi-index content determination methods of 35 batches of I. cappa were established. Eight of 17 common peaks of the liquid chromatographic fingerprints of I. cappa were identified. The results showed that the similarity was 0.898-0.997, eight components of which were used as indicators to determine the content of chlorogenic acid, neochlorogenic acid, cryptochlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, cynarin and luteolin, which were 0.353-3.765, 0.056-0.495, 0.086-0.526, 0.306-2.526, 0.861-7.353, 0.729-4.268, 0.052-0.424, 0.148-1.102 mg/g, respectively. Conclusion: The fingerprinting and multi-index content determination methods based on anti-inflammatory active ingredients established have high sensitivity, good accuracy, stability and reliability, which can be used for quantitative control of Miao medicine I. cappa.
