Effects of exosomes derived from MDA-MB-231 on proliferation of endothelial cells and the role of MAPK/ERK and PI3K/Akt pathways
- Author:
Shuang LONG
1
Author Information
1. Department of Pathophysiology, Institute of Stem Cell and Tissue Engineering, College of Basic Medicine, Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Akt;
Breast cancer;
Exosomes;
Extracellular signal regulatory kinase;
Proliferation
- From:
Medical Journal of Chinese People's Liberation Army
2012;37(11):884-888
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of exosomes derived from breast cancer cell line MDA-MB-231 on proliferation of human umbilical cord vein endothelial cells (HUVECs), and evaluate the role of MAPK/ERK and PI3K/Akt signal transduction pathway during the process. Methods Exosomes were derived and purified from MDA-MB-231 by cryogenic ultracentrifugation and density gradient centrifugation. MTT assay was carried out for measurement of cell proliferation in HUVECs with exosome of 50, 100, 200 and 400μJLg/ml. The states of cell cycle of HUVECs co-cultured with 200μJLg/ml exosomes were detected by flow cytometry. The effects of 200μJLg/ml exosomes on the expression of ERK, Akt and phosphorylated ERK, Akt in HUVECs were detected with Western blotting. Results Exosomes derived from MDA-MB-231 significantly promoted HUVECs proliferation in a classical time- and dose-dependent manner. Flow cytometry revealed that, co-cultured with 200μJLg/ml exosomes for 24h, S-phase cells in HUVECs increased, while G1/S phase cells in HUVECs decreased. Western blotting showed that, co-cultured with 200μxg/ml exosomes for 24h, 48h and 72h, the expressions of phosphorylated ERK and Akt were up-regulated in a time-dependent manner. Conclusion Exosomes derived from breast cancer cell line MDA-MB-231 may promote HUVECs proliferation, the changes in cell cycle and the continuous activation of the MAPK/ERK and PI3K/Akt signal transduction pathways may be the underlying mechanism.
