Expression of PTPeta in human hepatocellular carcinoma tissue and SMMC7721 cells and its significance
- Author:
Xiao-bing XU
1
Author Information
1. Department of Gastroenterology, Nanjing General Hospital of Nanjing Command
- Publication Type:Journal Article
- Keywords:
Cell adhesion;
Liver neoplasms;
Protein-tyrosine-phosphatase
- From:
Medical Journal of Chinese People's Liberation Army
2012;37(10):800-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of protein tyrosine phosphatase eta (PTPeta) in hepatocellular carcinoma tissue and SMMC-7721 cells, and observe the effects of SMMC7721 cell density on PTPeta expression. Methods Immunohistochemistry method was used to detect the protein expression of PTPeta in hepatocellular carcinoma tissues and SMMC-7721 cells. RT-PCR was employed to detect the mRNA expression of PTPeta in different growth density of SMMC-7721 cells (1×103, 5×103, 1×104, 5×104/cm2). Results Immunohistochemical detection demonstrated that the PTPeta was positively expressed in hepatocellular carcinoma tissue. Immunofluorescence study showed that PTPeta was also expressed in SMMC-7721 cells, and it was mainly localized in cell membrane. RT-PCR study indicated that the degree of expression of PTPeta mRNA was 0.425±0.031, 0.659±0.041, 0.771±0.043 and 0.885±0.045 respectively in cell density of 1×103, 5×103, 1×104 and 5×104/cm2. The expression of PTPeta mRNA was increased along with increase of tumor cell density with statistically significant difference (P<0.05). Conclusion PTPeta may by expressed in hepatocellular carcinoma tissue and tumor cells, and the expression may be increased by an increase in cell density. PTPeta may play an important role in proliferation and migration of malignant tumor cells by increasing the adhesiveness of hepatocarcinoma cells and maintaining the homeostasis of malignant tumor cells.
