Effect of simulated high-altitude hypoxia on Porphyromonas gingivalis
- Author:
Jing-jing HUANG
1
Author Information
1. Department of Stomatology, Xinqiao Hospital, Third Military Medical University
- Publication Type:Journal Article
- Keywords:
Anoxia;
Dental plague;
Detection rate;
Endotoxin level;
Porphyromonas gingivalis
- From:
Medical Journal of Chinese People's Liberation Army
2012;37(4):318-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of simulated high-altitude hypoxia on the detection rate and endotoxin level of Porphyromonas gingivalis (Pg) of subgingival bacterial plagues in rabbit periodontitis models. Methods Forty male rabbits were randomly divided into four groups, namely, normoxia control group (group A1), normoxia experimental group (group A2), hypoxia control group (group B1), and hypoxia experimental group (group B2). Each group included 10 rabbits. Periodontitis models was established in groups A2 and B2 combined by ligating both lower central incisors with steel ligature and feeding periodontitis diets, and then the animals were housed in a hypoxia chamber (simulating 5000m altitude, 23h per day). Groups A1 and A2 were raised normal diet in normoxia environment. After eight weeks, the rabbit periodontitis model was evaluated by observing radiographic features of the X-ray films and histopathologic changes under a light microscope. Subgingival plague sample from periodontal pockets on both lower central incisors were collected for isolation, culture and identification of Pg, and for detection of the endotoxin level. Results The histopathologic observation and X-ray examination results showed that the periodontitis of rabbits in group B2 was significantly more severe than that in group A2. The detection rates of Pg in group A1, A2, B1 and B2 was 0%, 50%, 55% and 95% (P<0.05). Pg detection rate and endotoxin level were higher in group B2 (95%, 0.46 0.04EU/ml) than in group A2 (50%, 0.38x 0.02EU/ml, P<0.05). Conclusions The process speed and damage degree of periodontitis in hypoxic environment is higher than that in normoxic environment. Moreover, the hypoxic environment is more suitable in the colonization of Pg with higher endotoxin level in subgingival plague.
