Deletion breakpoint mapping on chromosome 9p21 in breast cancer cell line MCF-7
- Author:
Hua-ping XIE
1
Author Information
1. Department of Gastroenterology, Huazhong University of Science and Technology
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Cell line;
Chromosome deletion;
MCF-7
- From:
Medical Journal of Chinese People's Liberation Army
2012;37(5):354-357
- CountryChina
- Language:Chinese
-
Abstract:
Objective To map the deletion breakpoint of chromosome 9p21 in breast cancer cell line MCF-7. Methods The deletion of chromosome 9p21 was checked by Multiplex Ligation-dependent Probe Amplification (MLPA) in MCF-7. Subsequently, the deletion breakpoint was amplified by long range PCR and the deletion region was narrowed by primer walking. Finally, the deletion position was confirmed by sequencing. Results The deletion was found starting within the MTAP gene and ending within CDKN2A gene by MLPA. Based on long range PCR and primer walking, the deletion was confirmed to cover the region from chr9:21819532 to chr9:21989622 by sequencing, with a deletion size of 170kb, starting within the intron 4 of MTAP and ending within the intron 1 near exon 1β of CDKN2A. Conclusions Long range PCR is an efficient way to detect deletion breakpoints. In MCF-7, the deletion has been confirmed to be 170kb, starting within the MTAP gene and ending within the CDKN2A gene. The significance of the deletion warrants further research.
