Protective effect of N-acetylcysteine on lung dendritic cells against damage in multiple organ dysfunction syndrome model
- Author:
Hong-wei WANG
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Dendritic cells;
Multiple organ failure;
N-acetylcysteine
- From:
Medical Journal of Chinese People's Liberation Army
2012;37(10):788-791
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the protective effect of N-acetylcysteine (NAC) on lung dendritic cells (DCs) from damage in multiple organ dysfunction syndrome (MODS) mouse model. Methods Animal model of MODS was established by injecting zymosan into the peritoneal cavity of BALB/c mice, and the mice were thereafter randomly divided into zymosan group, zymosan + NAC group and control group, 20 for each. MPO activity in lung tissue was measured by biochemical analysis 48 hours after modeling. Pathological changes of the lung were observed under light microscope. Lung DCs were separated by density gradient centrifugation and CD11c+ immunomagnetic beads. DCs' phenotypes of MHC-II/IAd and CD86 were analyzed by flow cytometry. Apoptosis of DCs was detected by flow cytometry with double labeling of Annexin V and 7-AAD. Results Compared with control group, the MPO activity in lung tissue remarkably increased in zymosan group (P<0.05). Infiltration by a large number of neutrophilic granulocytes was found in lungs, suggesting serious injuries in lung. The expressions of MHC-II/I-Ad and CD86 on DC surface and the percentage of DC apoptosis increased dramatically (P<0.05). Compared with zymosan group, the MPO activity remarkably declined and the lung injury was mitigatory (P<0.05); the number of infiltrating neutrophilic granulocytes decreased greatly, and the expressions of MHC-II/I-Ad and CD86 on the DC surface and the percentage of DC apoptosis decreased dramatically (P<0.05) in zymosan + NAC group. Conclusion NAC in vivo could inhibit DC activation-induced apoptosis and relieve lung injury, thus being of protective effect on lung DCs against damage in MODS model.
