Inflammatory activity of endothelial cells activated by intestinal lymph in severe heat stroke
10.11855/j.issn.0577-7402.2014.10.06
- Author:
Hua-Sheng TONG
1
Author Information
1. Department of ICU, Key Laboratory of Tropical Zone Trauma Care and Tissue Repair of PLA, Guangzhou General Hospital of Guangzhou Command
- Publication Type:Journal Article
- Keywords:
Endothelium;
Heat stroke;
Inflammation;
Lymph;
Vascular
- From:
Medical Journal of Chinese People's Liberation Army
2014;39(10):791-794
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of mesenteric lymph from rats suffering from heat stroke (SHS) on the inflammatory activity of vascular endothelial cells. Methods Rats were placed in a prewarmed incubator to reproduce heat stroke. Human umbilical vein endothelial cells (HUVECs ECV-340) were incubated in 5% mesenteric lymph collected pre-, during-, and post-HS for 3 and 6 h respectively in vitro. The levels of high mobility group protein B1 (HMGB1), tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), and IL-6 of ECV-340 cells were determined. The expression of intercellular adhesion molecule 1 (ICAM-1) mRNA and NF-κB activity of ECV-340 were also assayed. Results The levels of HMGB1, TNF-α, IL-1β and IL-6 induced by SHS mesenteric lymph of rats significantly increased after heat treatment (P<0.05), and this effect was enhanced with prolongation of exposure time (P<0.05). The expression of ICAM-1 mRNA and NF-κB activity were significantly elevated after being activated by SHS mesenteric lymph (P<0.05). Conclusion HS mesenteric lymph probably activates the inflammatory responses of vascular endothelium, which is closely associated with the pathogenesis of SHS.
