Effect of hydrogen sulfide on collagen synthesis in rat cardiomyocytes through down regulation of TGF-β1/Smad2/3 signaling pathway
10.11855/j.issn.0577-7402.2014.09.01
- Author:
Bai-Chuan LIU
1
Author Information
1. Department of Emergency Medicine, Beijing Jishuitan Hospital
- Publication Type:Journal Article
- Keywords:
Cardiac;
Hydrogen sulfide;
Myocytes;
Smad signal pathway;
Transforming growth factor β1
- From:
Medical Journal of Chinese People's Liberation Army
2014;39(9):681-684
- CountryChina
- Language:Chinese
-
Abstract:
Methods: Rat myocardial cell line (H9C2) was divided into four groups: normal control group (DMEM medium was used), NaHS group (NaHS at concentration of 2×10–4mol/L was used), TGF-β group (TGF-β1 at a final concentration of 10ng/ml was added) and TGF-β+NaHS group (cultured with 2×10–4mol/L NaHS for 30min, and then 10ng/ml TGF-β1 was added). After cultivation for 1h, p-Smad2 and p-Smad3 expressions in cardiomyocytes were assessed with Western blotting. After cultivation for 72h, collagen type expression was determined by immunofluorescence and confocal microscopy.
