Effects of ovariectomy on autophagy and senile plaques in the brain of adult APP/PS1 double transgenic mice
10.11855/j.issn.0577-7402.2015.01.06
- Author:
Ming-Liang XU
1
Author Information
1. Institute of Neuroscience
- Publication Type:Journal Article
- Keywords:
Alzheimer disease;
Autophagy;
Mice;
Ovariectomy;
Senile plaques;
Transgenic
- From:
Medical Journal of Chinese People's Liberation Army
2015;40(1):26-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ovarian estrogen on autophagy and senile plaques (SP) in the brain of adult APP/PS1 double transgenic mice, and inquire into the mechanism of Alzheimer disease (AD) neuropathogenesis due to estrogen deficiency. Methods APP/PS1 AD mice (3-month old) were divided into ovariectomy group (OVX-AD) and sham group (sham-AD). Two months after operation, transmission electron microscopy (TEM) was employed to observe the morphological structures of the brains of AD model mice, and immunohistochemical staining was performed to examine the changes in SP and autophagy-related proteins LC3 and Beclin-1 in the brain of AD model mice. Results Immunohistochemical staining showed that the number of SP in the brain of OVX-AD mice incrcased significantly compared with that in sham-AD mice, and distribution of SP was observed more extensively, including the cortex and hippocampus. The autophagy related proteins LC3 and Beclin-1 positive neurons were decreased greatly in the OVX-AD mice brain compared with that of sham-AD mice. TEM revealed a larger number of swollen and dark neurons in hippocampus of OVX-AD mice, while more autophagosome was observed around the neuronal processes in OVX-AD mice brain compared with sham-AD mice. Conclusion Estrogen deficiency may lead to delayed maturation of autophagy, decrease the activity and weaken the function of autophagy, which in turn may result in aggravation of pathological features of AD.
