Protective effect of adiponectin on bone marrow mesenchymal stem cell injury caused under fluid shear stress
10.11855/j.issn.0577-7402.2016.10.02
- Author:
Chong-Xia LIU
1
Author Information
1. Department of Geriatrics, Xijing Hospital, Fourth Military Medical University
- Publication Type:Journal Article
- Keywords:
Adiponectin;
Mesenchymal stem cells;
Shear stress;
Tissue engineering
- From:
Medical Journal of Chinese People's Liberation Army
2016;41(10):798-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore whether adiponectin (APN) can attenuate the injury of bone marrow mesenchymal stem cells (BMSCs) caused under fluid shear stress (SS) and the mechanism involved. Methods BMSCs were isolated and cultured by whole blood adherent method, and then identified and divided into control group, SS (10dyne/cm2) group and SS+APN group. BMSCs were subjected to fluid SS for 24 hours by using liquid plate flow chamber model, the cell morphology was observed by microscopy, the apoptosis of BMSCs was detected by TUNEL staining, and Western blotting was performed to detect the protein expressions of Akt, p-Akt and Bax. Results Compared with the control group, the apoptosis of BMSCs significantly increased, and the expression of Bax was up regulated in SS group. Compared with SS group, the cell morphology was significantly improved, the number of cells obviously increased, the Bax expression was down-regulated and the p-Akt expression was up-regulated markedly in SS+APN group. There was no significant difference in Akt expression among the three groups, but the p-Akt expression significantly increased in SS+APN pretreatment group. Conclusion Adiponectin may activate the Akt signaling pathway and inhibit the apoptosis of BMSCs under fluid SS.
