Effect of TGF-β1 on epithelial mesenchymal transformation and invasion of choriocarcinoma cells
10.11855/j.issn.0577-7402.2016.02.06
- Author:
Yan-Jie LU
1
Author Information
1. Institute of Cancer Research, Chengde Medical College
- Publication Type:Journal Article
- Keywords:
Cadherins;
Cell movement;
Choriocarcinoma;
Neoplastic stem cells;
Transforming growth factor beta 1
- From:
Medical Journal of Chinese People's Liberation Army
2016;41(2):114-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of TGF-β1 on epithelial mesenchymal transformation and invasion by promoting cancer stem cell marker CD133 expression in choriocarcinoma cells. Method Choriocarcinoma cells JEG-3 were cultured in vitro and incubated with TGF-β1 at different time and concentration, and the expression of CD133 protein and mRNA of EMT markers were detected by Western blotting and PCR respectively. The effect of TGF-β1 on invasive ability of JEG-3 cells were assessed with transwell method. Results TGF-β1 promoted the expression of cancer stem cell marker CD133, downregulated the epithelial marker E-cadherin, upregulated mesenchymal marker N-cadherin, and promoted invasion ability in choriocarcinoma cells. Conclusion TGF-β1 could promote stem cell property of cancer, EMT property, and invasive property of choriocarcinoma cells.
