The hemostatic property of calcium alginate sponge: An experimental study
10.11855/j.issn.0577-7402.2016.03.02
- Author:
Jie ZONG
1
Author Information
1. College of Food Science and Engineering, Shanghai Ocean University
- Publication Type:Journal Article
- Keywords:
Absorbable;
Calcium alginate;
Cell toxicity;
Gelatin sponge;
Hemostatics
- From:
Medical Journal of Chinese People's Liberation Army
2016;41(3):180-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the hemostatic property of calcium alginate (CA) sponge and its degree of safety on exterior use. Methods The optimal coagulation concentration of CA was determined by Lee-White clotting test, and then the CA was freeze-dried to form a sponge. The coagulation effect in vitro of the CA sponge was determined by Blood Clotting Index (BCI) test. The ear artery bleeding model and full-thickness skin wound model of rabbit were employed to determine the hemostatic property of CA sponge. The degree of safety of CA sponge was evaluated by cell toxicity test. Results The BCI was significantly decreased in CA sponge group (33.08±4.02) than that in gelatin sponge (72.05±10.48, P<0.05). In rabbit ear artery bleeding test, the volume of blood loss was obviously less in CA sponge group (2.85±0.14g) than that in gelatin sponge group (2.97±0.17g) or hospital gauze group (3.93±0.18g, P<0.05). In rabbit full-thickness skin wounds test, the total amount of blood absorbed was lower in CA sponge group (2.66±0.24) than in hospital gauze group (3.81±0.73, P<0.05), but the hemostasis time in CA sponge group (97.83±5.49s) showed no significant difference as compared with that in gelatin sponge group (80.25±7.41s, P>0.05), though they were all shorter significantly than that in hospital gauze group (101.00±14.71s, P<0.05). The cell toxicity of 100% CA sponge was level 1, and it complied with the safety requirements for biomaterials. Conclusion The CA sponge has a good hemostatic property without obvious cytotoxicity.
