Evaluation of age-related changes of early kidney injury markers in plasma of septic rats
10.11855/j.issn.0577-7402.2019.06.03
- Author:
Sheng-Mao JIANG
1
Author Information
1. Chinese PLA Medical School
- Publication Type:Journal Article
- Keywords:
Acute kidney injury;
Biomarker;
Sepsis
- From:
Medical Journal of Chinese People's Liberation Army
2019;44(6):466-470
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate age-related changes of early plasma renal injury markers in rats with sepsis, which can serve as evidence for the early detection of acute kidney injury (AKI). Methods Twenty-one male Sprague Dawley rats were divided into 3 groups based on their age: young group(12 weeks, n=7), adult group (12 months, n=7), and old group (D-galactose-induced 12 months, 500 mg/(kg.d), for 6 weeks, n=7). All rats were received cecal ligation and puncture (CLP) procedure to induce sepsis. Plasma samples of each rat were collected 0.5 h before CLP, and 0.5 h, 4.5 h, 8.5 h after CLP. For each sample, plasma level of kidney injury markers, including plasma creatinine (Cr), blood urea nitrogen (BUN), neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), interleukin-6 (IL-6), Cystatin C (CysC), insulin-like growth factor-binding protein 7 (IGFBP-7), tissue inhibitor of metalloproteinases-2 (TIMP-2) and [TIMP-2]*[IGFBP-7] were quantified. We adopted the criteria for AKI: plasma creatinine elevation =1.5 times of the baseline level. Within-group longitudinal changes and between-group changes were evaluated. Results Compared with samples collected at 0.5 h before CLP, plasma Cr levels were significantly increased at 0.5 h, 4.5 h and 8.5 h after CLP in all three groups (P<0.05), while plasma IL-6 [(121.1±14.9) pg/ml vs. (98.24±13.37) pg/ml, P=0.009] and BUN [(5.43±0.73) mmol/L vs. (5.06±0.82) mmol/L, P=0.041] significantly decreased at 4.5 h after CLP in adult rats, Only the young rats suffered AKI. In between-group comparison, plasma level of IL-6 [(98.24±13.37) pg/ml vs. (116.86±19.75) pg/ml, P=0.046], TIMP-2 [(4042.56±781.68) pg/ml vs. (5662.57±1527.8) pg/ml, P=0.013], [TIMP-2]*[IGFBP-7] [(0.112±0.03) (ng/ml)2/1000 vs. (0.17±0.052) (ng/ml)2/1000, P=0.021] significantly increased at 4.5 h after CLP and BUN [(5.34±0.43) mmol/L vs. (6.29±0.51) mmol/L, P=0.017] at 8.5 h after CLP in the young group, compared with the adult rats. There was no significant difference in the plasma levels of other markers when compared with 0.5 h before CLP and also no significant between-group difference (P>0.05). Conclusion Only the young rats suffered earlier and more serious sepsis-induced AKI. Plasma level of Cr may be useful for early detection of sepsis. Age may not be a risk factor for early AKI in sepsis. Plasma TIMP-2, [TIMP-2]*[IGFBP-7] are potential markers for the detection of early AKI in sepsis in both young and adult animals.