Expression of TIM-3 in PBMCs and its relation to treg in the patients with allergic rhinitis
10.11855/j.issn.0577-7402.2019.07.13
- Author:
Zi-Qin XU
1
Author Information
1. Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Jinzhou Medical University
- Publication Type:Journal Article
- Keywords:
Allergic rhinitis;
TIM-3;
Treg
- From:
Medical Journal of Chinese People's Liberation Army
2019;44(7):611-614
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the expression of TIM-3 (T cell immunoglobulin domain, mucin domain) and its relationship with Treg (regulatory T) cells isolated from peripheral blood mononuclear cells (PBMCs) in the patients with allergic rhinitis (AR), and investigate the role of TIM-3 in the occurrence and development of the allergic rhinitis inflammation. Methods AR patients (AR group, n=30) and healthy subjects (HC group, n=25) were selected as experimental group and control group respectively, and 2 ml peripheral venous bloods were collected from these two groups. Peripheral blood mononuclear cells (PBMCs) were isolated from the blood by density gradient centrifugation. The proportion of CD4+CD25+FoxP3+, reflecting expression of Treg, and TIM-3+Treg was detected by flow cytometry. The relationship between TIM-3 expression and Treg cells was analyzed. The expression of TIM-3 on the surface of CD4+CD25+FoxP3+ Treg cells was analyzed by flow cytometry. Then the TIM-3 expression was blocked, and its influence on Treg cells was observed. Results The percentage of Treg cells in peripheral blood of patients with AR (1.16%±0.13%) was lower than that of healthy controls (5.12%±0.11%) (Z=–6.339, P<0.01). The expression of TIM-3 on the surface of Treg cells in AR patients (11.76%±1.07%) was higher than that in healthy controls (3.15%±0.22%) (Z=–5.570, P<0.01). The expression of CD4+CD25+FoxP3+Treg cells in AR patients was negatively correlated with the expression of TIM-3 on the surface (r=–0.763, P<0.05); The percentage of Treg cells in peripheral blood of AR patients after the block (1.67%±0.76%) was significantly higher than that before block (1.44%±0.78%) (Z=–1.45, P=0.135). The percentage of IL-10 secreted by Treg cells in peripheral blood after block (2.33%±1.45%) was higher than that before block (1.57%±1.12%) (Z=–2.97, P=0.003) in AR patients. Conclusion The expression of TIM-3 in PBMCs may increase in patients with AR, TIM-3 may play an important role in the pathogenesis of AR, and may be related to the imbalance of Treg cells.
