Establishment of different lung impact injury models in adult rats
10.11855/j.issn.0577-7402.2019.08.03
- Author:
Dan-Feng YUAN
1
Author Information
1. Institute of Surgery Research, Daping Hospital, Army Medical University
- Publication Type:Journal Article
- Keywords:
Adult rats;
Animal model;
Biological shock tube;
Lung blast injury
- From:
Medical Journal of Chinese People's Liberation Army
2019;44(8):643-647
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an adult rat model of lung blast injury in laboratory for providing a reliable rat model for the diagnosis and treatment of lung blast injury. Methods: According to the different injury driving pressure of BST-I biological shock tube, 40 adult SD male rats aged 7 to 8 weeks were randomly divided into 4 groups (10 each): 4.5 MPa group, 4.8 MPa group, 5.0 MPa group and 5.5 MPa group. The general physiological and 24-hour survival status of rats in each group were recorded before and after modeling. The survived rats 24 h after modeling were anesthetized and then killed, and the chest anatomy was performed. The characteristics of lung injury were observed, the degree of lung tissue injury was graded using organ injury scaling (OIS) and abbreviated injury scale (AIS). Furthermore, the left lower part of lung tissue was taken for routine pathological section, and the degree of lung tissue injury was observed under light microscope. Results: All rats were successfully modeled and survived 24 h after injury, and showed shortness of breath and accelerated heart rate without hematuria and blood stool. There were significant differences among the four groups in bleeding rate of external auditory canal, anal temperature and pulmonary bleeding area (P<0.01). The grading scores of lung injury degree in four groups were as follows: for 4.5 MPa group, the OIS grade concentrated in grade II, the AIS score concentrated in score 1; for 4.8 MPa group, the OIS grade concentrated in grade HI, the AIS score concentrated in score 2; for 5.0 MPa group, the OIS grade concentrated in grade IV, the AIS score concentrated in score 3; for 5.5 MPa group, the OIS grade concentrated in grade V, and the AIS score concentrated in score 4. There existed significant differences in grading and score among the four groups (P<0.05). However, the results of OIS classification showed that the lung injury grades of 5.0 MPa group and 5.5 MPa group were higher than that of 4.5 MPa group, and the grade of 5.5 MPa group was higher than that of 4.8 MPa group. As to AIS score, statistical differences existed between any two groups on the lung tissue injury score (P<0.05), and the AIS scores ranged from high to low were as follows: 5.5 MPa>5.0 MPa>4.8 MPa>4.5MPa. Under light microscope, rats in the four groups showed varying degrees of pulmonary hemorrhage, edema and atelectasis, and the lung tissue was ruptured. Conclusion: The stable adult SD rat models of mild, moderate, severe and extremely severe lung blast injury have been established.
