Relationship of IL-37 in the serum and peritoneal fluid to TNF-α and IFN-γ in endometriosis patients
10.11855/j.issn.0577-7402.2020.03.13
- Author:
Zhao-Ying JIANG
1
Author Information
1. Department of Obstetrics and Gynecology, Third Xiangya Hospital of Central South University
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Interferon-γ;
Interleukin-37;
Tumor necrosis factor-α
- From:
Medical Journal of Chinese People's Liberation Army
2020;45(3):304-307
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) to interleukin-37 (IL-37) in the serum and peritoneal fluid, and investigate the pathogenesis of IL-37 in endometriosis (EMT) patients. Methods Thirty-six EMT patients, admitted in the Department of Obstetrics and Gynecology, the Third Xiangya Hospital of Central South University from Jul, 2018 to Jan. 2019, and confirmed by laparoscopic operation and pathology, were selected as the EMT group, of which 13 cases were in stage I and II, and 23 cases in stage III and IV. The other 28 women with simple ovarian cyst, teratoma or hysteromyoma were selected as the control group. ELISA was performed to detect the levels of IL-37, TNF-α and IFN-γ in serum and abdominal fluid of the two groups. And then the levels of IL-37, TNF-α and IFN-γ were compared between the two groups and their relationship was analyzed. Results The serum levels of IL-37 and TNF-α were significantly higher in EMT group than those in control group [(101.78±31.58) pg/ml vs. (63.50±14.48) pg/ml and (30.68±16.22) pg/ml vs. (18.09±1.27) pg/ml, respectively]. The levels of IL-37 and TNF-α in peritoneal fluid were significantly higher in EMT group than those in control group [(133.94±47.07) pg/ml vs. (68.43±12.60) pg/ml and (24.23±6.12) pg/ml vs. (16.63±3.13) pg/ml, respectively]. The levels of IFN-γ in serum and peritoneal fluid were significantly lower in EMT group than that in control group [(18.81±3.20) pg/ml vs. (20.52±1.38) pg/ml and (15.93±2.58) pg/ml vs. (20.32±7.03) pg/ml, respectively]. The differences were statistically significant (P<0.05). In EMT group, the expressive levels of IL-37 and TNF-α increased, and of IFN-γ decreased in serum and peritoneal fluid with the aggravation of the disease. In EMT group, the IL-37 levels in serum and peritoneal fluid were positively correlated with the TNF-α levels (r=0.338 and 0.518 respectively, P<0.05), while negatively correlated with the IFN-γ levels (r=–0.332 and –0.370 respectively, P<0.05). In control group, no significant correlation existed in IL-37 to TNF-α or to IFN-γ in serum and peritoneal fluid (P>0.05). Conclusion The levels of IL-37 in serum and peritoneal fluid are increased significantly in EMT patients, implying that IL-37 may play a certain role in occurrence and progression of EMT by promoting secretion of TNF-α and inhibiting secretion of IFN-γ.
