Study on the correlation of HMGB1 and coagulation disorder in severe heatstroke rats
10.11855/j.issn.0577-7402.2020.03.11
- Author:
Bao-Jun YU
1
Author Information
1. Department of Intensive Care Medicine, Affiliated Shenzhen Baoan Hospital of Southern Medical University
- Publication Type:Journal Article
- Keywords:
Coagulation disorder;
High-mobility group protein B1;
Severe heatstroke
- From:
Medical Journal of Chinese People's Liberation Army
2020;45(3):292-297
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association between HMGB1 and coagulation disorder in severe heat stroke rats. Methods A total of 48 rats were randomized equally into 8 groups: Room temperature group (Sham), severe heatstroke (HS) re-cooling 0 h (HS-0 h), 3 h (HS-3 h), 6 h (HS-6 h), 9 h (HS-9 h), 12 h (HS-12 h), 18 h (HS-18 h), 24 h (HS-24 h) groups. Sham group rats were housed at room temperature of (25.0±0.5) ℃ and humidity of (50.0%±5.0%), while severe heatstroke group rats were kept in an incubator at a temperature of (39.5±0.2) ℃ and humidity of 60.0%±5.0%. Rats with the rectal temperature (Tr) reached 43 ℃ were defined as the onset of severe heatstroke, followed by transferring to the room temperature for natural cooling. The blood samples were collected at 0, 3, 6, 9, 12, 18 and 24 h after natural cooling. Prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (Fib) were evaluated by clotting methods. HMGB1and thrombin-antithrombin (TAT) were detected by ELISA. Results A linear association between HMGB1 and PT was found (P<0.05); a linear association between HMGB1 and TAT was found (P<0.001); the linear relationship between HMGB1 and TT or APTT was found, but there was no statistic difference (P>0.05); a nonlinear association between HMGB1 and PLT was found (P<0.001). There was no association between HMGB1 and Fib (P>0.05). Conclusions HMGB1 has a significant association with coagulation disorder in severe heat stroke rats. The mechanism needs to be further studied.
