Inhibition of endotoxin on the function of cardiac sarcoplasmic reticulum in rats
10.11855/j.issn.0577-7402.2020.07.03
- VernacularTitle: 内毒素对大鼠心肌肌质网功能的抑制作用
- Author:
Huai-Sheng CHEN
1
Author Information
1. Department of Intensive Care Unit, Shenzhen Peoples’ Hospital
- Publication Type:Journal Article
- Keywords:
Calsequestrin;
Cardiomyocytes;
Endotoxin;
Sarcoplasmic reticulum Ca2+-ATPase;
Sodium-calcium exchanger
- From:
Medical Journal of Chinese People's Liberation Army
2020;45(7):697-701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the enzymatic changes of myocardial sarcoplasmic reticulum in rats after injecting endotoxin (LPS), and provide basic research results for the future study of myocardial sarcoplasmic reticulum dysfunction caused by LPS in rats. Methods Ten SD rats were randomly divided into blank control group and LPS injection group with 5 rats in each group. In the LPS injection group, endotoxin was injected into the tail vessels of the rats. Results The heart rate (HR) of the LPS injection group increased and was faster than that in the blank control group [(204±18) beat per min vs. (139±10) beat per min on the first day, and (199±22) beat per min vs. (143±17) beat per min on the next day, both P values were less than 0.05]. The mean arterial pressure (MAP) was lower than that of the blank control group on the first day [(87±12) mmHg vs. (102±7) mmHg, P<0.05]. Under light and electron microscope, the myocardial cells of rats with LPS injection were loosely arranged, with intercellular infiltration with inflammatory cells, muscle fibers broken, and difficult to identify the morphology of mitochondria and sarcoplasmic reticulum. Quantitative PCR results showed that after endotoxin injection, troponin (CASQ1), sodium-calcium exchanger (NCX), calmodulin phosphatase 1 (ppplCa), phospholipid protein (PLN), sarcoplasmic reticulum Ca2+-ATPase (SERCA2) increased significantly (P<0.05). Conclusion Endotoxin can inhibit cardiomyocyte function by affecting the activity of sarcoplasmic reticulum calcium regulatory protein-related enzymes through various mechanisms.
