Nude mouse model of postoperative metastasis of human lung cancer and dynamic observation
- Author:
Lei LIU
1
Author Information
1. Department of Life Science
- Publication Type:Journal Article
- Keywords:
Carcinoma non-small-cell lung;
Immunohistochemistry;
Mice, nude;
Models, animal;
Neoplasm metastasis
- From:
Tumor
2007;27(4):265-268
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a nude mouse model of postoperative metastasis of human lung cancer and perform dynamic observations, so as to provide a reliable animal model to study the metastatic mechanism and investigate the postoperative anti-metastasis treatment. Methods: Subcutaneous transplanted model of non small cell lung carcinoma (NSCLC) NCI-H460 cells was established by tissue explant technique. Four weeks later, 25 nude mice were randomly selected and had tumor resection to establish the postoperative metastasis model. The other 15 nude mice were regarded as control. Then five mice in each group were sacrificed every two weeks to make the dynamic observation of the distant metastasis. The experiments were terminated when the body weight of nude mice were obviously decreased. The expression of the matrix metalloproteinase-2 (MMP-2), osteopontin (OPN), CD44v6, and CD62 in metastases and primary lesions were detected by immunohistochemical method. Results: The tumor body had severe necrosis and the body weight of mice had apparently decrease in the control group after 10 weeks. No metastasis was found in each stage. While in the operation group, metastasis was found in one of the five sacrificed nude mice at the 10th and 12th week. The metastasis rate was 20%. The lung metastasis rate reached 100% (5/5) with apparent visible lung metastatic nodes when the mice were sacrificed at 14th week. But no metastasis was observed in other organs. Immunohistochemical (IHC) examination showed that the expression of MMP-2, OPN, CD44v6, CD62, E-cadherin, and Timp-2 in metastases were markedly higher than that in primary tumor, however the expression of CD54 and MMP-9 in metastases were significantly lower than that in primary tumor. Conclusion: The model simulates the tumor distant metastasis process after clinical operation. The results of IHC indicate that the metastasis might correlate with abnormal expression of some adhesion molecules. It provides a valuable model for studying the metastatic mechanism and investigating the postoperative anti-metastasis treatment.
