Detection of bladder cancer by accessing DNA methylation state in urine sediments
- Author:
Jun MENG
1
Author Information
1. Department of Urology
- Publication Type:Journal Article
- Keywords:
Bladder neoplasms;
DNA Methylation;
Methylation specific polymerase chain reaction;
Urinalysis
- From:
Tumor
2007;27(5):374-378
- CountryChina
- Language:Chinese
-
Abstract:
Objectives: To assess DNA methylation state of the 13 genes in urine sediments and evaluate their value in diagnosis of bladder cancer. Methods: Ninety-two patients with bladder cancer, 23 patients with non tumorous urinary disease, 6 patients with neurological disease, and 7 healthy volunteers were recruited in the study. The DNA methylation state of tumor-related promoters in urine sediments was determined by Methylation specific PCR method (MSP). Results: The frequency of hypermethylation of 13 genes in 92 cases with bladder cancer was higher than that in 23 cases of bladder diseases, while all the genes were at demethylation state in the 7 healthy volunteers and 6 patients with neurological disease. The incidence of hypermethylation was significantly higher in bladder cancer compared with non tumorous urinary disease (P < 0.05). If DNA hypermethylation was used as a positive indicator, 88.0% (81/92cases) of bladder cancer could be detected. Conclusion: Assessing DNA methylation state in the promoter of tumor-related genes in urine sediments by MSP method is effective for detection of bladder cancer.
