Antisense oligonucleotide targeting survivin induces apoptosis of SMMC-7721 cell and enhances its sensitivity to chemotherapeutic agents in vitro
- Author:
Li PENG
1
Author Information
1. Department of Hepatobiliary Surgery
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Carainoma, hepatocellular;
Drug resistance, neoplasm;
Oligonucleotides, antisense;
Survivin
- From:
Tumor
2007;27(5):361-364
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the effect of antisense oligonucleotide (ASODN) targeting survivin on apoptosis, proliferation and sensitivity to chemotherapeutic agents of hepatocellular carcinoma cell line SMMC-7721. Methods: Specific ASODN targeting survivin was designed and constructed, and transfected into SMMC-7721 cells mediated by oligofectamine. Cell morphological changes were examined by transmission electron microscopy. The expression of survivin mRNA was detected by RT-PCR. Cell cycle, apoptosis and the expression of survivin protein were detected by flow cytometry. Changes of sensitivity to pirarubicin, doxifluridine, and cisplatin of SMMC-7721 cells were detected by MTT assay. Results: Morphological abnormalities of cells were observed in ASODN transfected groups. The mRNA and protein expressions of survivin were significantly decreased compared with that of control group (P <0.05). The transfection of survivin ASODN induced significant apoptosis (P <0.01) and increased the proportion of cells in the G2/M phase(P < 0.05). Compared with that in control group, oligofectamine group and SODN groups, the sensitivity of ASODN groups to pirarubicin, doxifluridine, and cisplatin were significantly enhanced (P <0.01). Conclusion: The transfection of survivin ASODN down-regulated the mRNA and protein expressions of survivin, induces apoptosis, and enhances its sensitivity to pirarubicin, doxifluridine, and cisplatin of SMMC-7721 cells.
