The effect of DLX4 on the apoptosis of choriocarcinoma cells and its related mechanism
- Author:
Yun-Yan SUN
1
Author Information
1. Obstetrics and Gynecology Hospital
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Choriocarcinoma;
DLX4;
RNA interference
- From:
Tumor
2007;27(5):357-360
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of homeodomain protein DLX4 on apoptosis of choriocarcinoma cells and its related mechanism. Methods: RNA interference (RNAi) was used to knockdown DLX4 expression in JEG-3 cells. Then the inhibitory effect of DLX4 on apoptosis was determined by flow cytometry and the expression of apoptosis-related protein cleaved caspase-3 and caspase-8 and bax was detected by western blotting. Results: RNA interference targeted for DLX4 effectively and specifically inhibited DLX4 mRNA expression and decreased DLX4 protein level in JEG-3 cells. The apoptotic rate of JEG-3 cells was increased, the expression levels of cleaved both caspase-3 and caspase-8 were elevated but the bax protein expression did not change after knocking down DLX4. Conclusion: RNA interference silences DLX4 expression and induces apoptosis of JEG-3 cells. The mechanism for apoptosis was related with transcriptional regulation on caspase-3 and caspase-8.
