Relation between ionization and the expressions of HIF-1α and VEGF in anoxia condition
- Author:
Hui-Hua CHEN
1
Author Information
1. Department of Radiotherapy
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor 1, alpha subunit;
Liver neoplasms, experimental;
Radiation, ionizing;
Vascular endothelial growth factor
- From:
Tumor
2007;27(7):545-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of ionization on the expression of HIF-1 α and VEGF in anoxia condition, in order to search an effective method for improving the radiosensitivity. Methods: HepG2 hepatoma carcinoma cells were divided into four groups: the control group; the hypoxia group; the radiation group; the hypoxia and radiation group. The cell apoptosis was detected by fluorescence microscope. The cell viability was analyzed by MTY method. And we measured the expressions of HIF-1 α mRNA and VEGF mRNA by RT-PCR. Results: Cell apoptosis: no apoptosis was observed in the control group; few apoptosis occurred in the hypoxia group; bulk apoptosis was observed in the radiation group; less apoptosis occurred in the hypoxia and radiation group than that in the hypoxia group. The order of surviving fraction was the control group > the hypoxic group > the hypoxia plus radiation group > the radiation group; The order of the expression of HIF-1 α mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group, no significant difference was observed between the radiation group and the control group; The order of the expression of VEGF mRNA: the hypoxia plus radiation group > the hypoxic group > the radiation group > the control group. Conclusion: The expression of HIF-1α induced by hypoxia can prevent hepatoma cells from the damage of radiation. The HIF-1 α decreases the radiosensitivity through inducing VEGF expression in the HepG2 cell line.
