The action mechanism of the effects of vitamin E succinate on apoptosis of human oral squamous cancer Tca 8113 cells
- Author:
Xiao-Dong SONG
1
Author Information
1. Department of Biology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Apoptosis regulatory proteins;
Carcinoma, squamous cells;
Tca 8113 cells;
Vitamin E succinate
- From:
Tumor
2007;27(7):542-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of vitamin E succinate (VES) on apoptosis of Tca 8113 human oral squamous carcinoma cells and its action mechanism. Methods: Flow cytometry was applied to determine the effect of VES on cell cycle distribution and apoptosis after PI staining or Annexin V/PI double staining. The expression of Bax mRNA was quantified by RT-PCR. The relative amounts of the Bax protein was measured by Western blotting. Results: VES significantly inhibited the growth of Tca8113 cells, induced typical apoptosis, and up-regulated the expression of Bax mRNA and protein. After 24 h the apoptotic rate reached the peak level. Conclusion: VES induces apoptosis of Tca8113 human oral squamous carcinoma cells, which is probably related with up-regulation of Bax expression.
