The experimental study on the anti-angiogenenic effect of honokiol
- Author:
Jia WANG
1
Author Information
1. Department of Bio-Medicine
- Publication Type:Journal Article
- Keywords:
Angiogenesis inhibitors;
Chorioallantoic membrane (CAM);
Honokiol;
Vascular endothelial growth factors
- From:
Tumor
2007;27(7):527-530
- CountryChina
- Language:Chinese
-
Abstract:
Objective: This study was designed to evaluate the in vivo anti-angiogenic effect of honokiol in chick chorilallantoic membrances (CAM), as well as its in vitro effect in human umbilical vein endothelial cells (HUVECs) and colorectal RKO cell line. Methods: HUVECs were cultured primarily in vitro, and the influence of honokiol on the proliferation of HUVECs, human fibroblasts and human colorectal RKO cells were evaluated by MTT assay. CAM model was used to check the inhibitory effect of honokiol on neovascularization in vivo. Vascular endothelial growth factor A (VEGF-A) mRNA expression was detected by reverse transcriptase polymerase chain reaction (RT-PCR) assay and VEGF protein level in supernatant was determined by enzyme-linked immunosorbent assay (ELISA). Results: Honokiol inhibited cell proliferation in HUVECs and RKO cells, but the inhibitory concentration 50% (IC50) was much lower in HUVECs (14.5 μmol/L) than that in primary human fibroblasts (150 μmol/L) and RKO cells (42 μmol/L). In CAM assay, honokiol dose-dependently inhibited the neovascularization, with inhibitory rate of 58% and 86% at the dosage of 0. 1 μg and 0.2 ♂g per cell respectively. Honokiol at 10 μmol/L and 20 μmol/L significantly inhibited VEGF-A mRNA expression and VEGF secretion in supernatants of cultured RKO cells. Conclusion: Honokiol inhibites angiogenesis at non apoptosis-inducing dosage both in vitro and in vivo. Its anti-angiogenic effect might be mediated by inhibiting endothelial cells proliferation as well as VEGF expression in tumor cells.
