The effect of low-dose cyclophosphamide chemotherapy on angiogenesis of lung carcinoma
- Author:
Ming LI
1
Author Information
1. Department of Respiratory Medicine
- Publication Type:Journal Article
- Keywords:
Hypoxia-inducible factor 1, alpha subunit;
Lung neoplasms;
Neovascularization, pathologic;
Vascular endothelial growth factors
- From:
Tumor
2007;27(7):523-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the efficacy of continuous low-dose cyclophosphamide (CTX) chemotherapy on the angiogenesis of lung carcinoma and the corresponding mechanism. Methods: Cultured A549 lung adenocarcinoma cells were transplanted into BALB/c nude mice. Three groups were randomly divided: low-dose CTX group, high-dose CTX group, and control group. The body weight of nude mice and the tumor growth were recorded. Tumor microvessel density (MVD) and the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 alpha (HIF-1 α) were detected by immunohistochemical staining. Results: The tumor growth was delayed and the side effects such as body weight loss was significantly relieved in low-dose CTX group compared with high-dose CTX group and control group (P < 0.05 or P < 0.01). The level of MVD and expression of HIF-1 α and VEGF were significantly decreased in low-dose CTX group than those in high-dose CTX group and control group (P < 0. 01). HIF-1α had significant correlation with VEGF and MVD (P < 0.05) and MVD was significantly associated with VEGF expression in low-dose CTX group (P < 0.05). Conclusion: Compared with high-dose CTX chemotherapy and normal saline therapy, the continuous low-dose CTX chemotherapy has higher efficacy in inhibiting the tumor growth and angiogenesis. The mechanism may be due to the down-regulation of HIF-1α expression.
