The expression of psiTPTE22 mRNA containing LTR sequence in tumors
- Author:
Qiao-Yi LIANG
1
Author Information
1. Cancer Institute
- Publication Type:Journal Article
- Keywords:
Gene expression regulation, neoplastic;
Gene, psiTPTE22;
Oligonucleotide array sequence analysis
- From:
Tumor
2007;27(8):633-637
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the mRNA levels of AK097082 and BC031001 encoded by psiTPTE22 gene in order to determine whether psiTPTE22 gene is the microarray-detected over-expressed gene in colon cancer and explore the role of psiTPTE22 in carcinogenesis. Methods: RT-PCR and real-time fluorescence quantitative PCR were used to detect the transcription of AK097082 and BC031001 encoded by psiTPTE22 gene in kidney, liver, stomach, lung, and colon cancer tissues and adjacent normal tissues as well as nine cancer cell lines and placenta tissues. Results: RT-PCR showed that only the mRNA of BC031001 encoded by psiTPTE22 was expressed in colon tissues. Real-time quantitative PCR assay and F test confirmed that there was no significant difference between colon cancer and adjacent normal tissues (P > 0.05). So psiTPTE22 was not the specific up-regulated gene in colon cancer detected by microarray. Real-time quantitative PCR assays also confirmed that BC031001 was over-expressed in kidney, liver, stomach, and lung normal tissues but had low expression in corresponding cancer tissues. BC031001 had no expression or weak expression in several cancer cell lines but had relatively high expression in placenta tissues. F tests found that the difference was significant in the expression level of BC031001 between kidney, liver, stomach, and lung adjacent normal tissues and the corresponding cancer tissues (P < 0.05). Conclusions: The mRNA expression of BC031001 encoded by psiTPTE22 gene has negative correlation with several kinds of cancer. Further studies are needed to reveal the mechanism underlying its down-regulation and its role in a carcinogenesis.
