Detection and significance of promoter hypermethylation of p16 gene and MGMT gene in plasma from non-small cell lung cancer patients
- Author:
Yun-Ming KONG
1
Author Information
1. School of Public Health
- Publication Type:Journal Article
- Keywords:
Carcinoma, non-small cell lung;
DNA methylation;
MGMT gene;
p16 gene
- From:
Tumor
2007;27(9):715-718
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the aberrant methylation in the promoter of p 16 and O6-methylguanine DNA methyltransferase (MGMT) gene in peripheral plasma from non-small cell lung cancer (NSCLC) patients and to evaluate the clinical significance in the screening and early diagnosis of NSCLC. Methods: A nested methylation-specific PCR (nMSP) was performed for the detection of promoter hypermethylation of p16 gene and MGMT gene in plasma from 65 NSCLC patients. Results: Of the 65 plasma samples, p16 gene promoter hypermethylation was detected in 19 patients (29.23%) and MGMT gene promoter hypermethylation was found in 16 patients (24.62%). However, promoter hypermethylation in p16 gene and MGMT gene was not found in plasma samples from the normal controls (P < 0.05). The hypermethylation detection rate in plasma of the two genes did not significantly correlate with the pathological classification and clinical staging of NSCLC (P >0.05). Conclusion: Detection of the aberrant methylation in the promoter of p16 gene and MGMT gene in plasma from NSCLC patients by nMSP method might provide valuable information for the screening, early diagnosis, and prediction of prognosis of NSCLC.
