Comparative study on the proteomic analysis in cervical carcinoma tissues with different radiosensitivities
- Author:
Liang ZENG
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Electrophoresis, gel, two-dimensional;
Proteomics;
Radiotherapy;
Spectrum analysis, mass;
Uterine cervical neoplasms
- From:
Tumor
2007;27(10):808-812
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To compare the proteomic differences in early cervical carcinoma tissues with various radiosensitivities and provide the basis for determining of radiosensitivity-associated proteins in cervical carcinoma. Methods: The fresh carcinoma tissues were collected from 10 untreated cervical carcinoma patients and preserved at - 80C. According to WHO Criterion of Therapeutical Effect of Solid Carcinoma, the tissues were classified into two groups: high sensitive group (HS) and low sensitive group (LS). The total protein was extracted and separated by using two-dimensional gel electrophoresis (2-DE). The PD-quest 7.0 software was used to perform image matching and difference analysis to identify the differentially expressed protein-spots between the two groups. The identified protein spot was cut in electrophoretic gel in situ, digested with trypsin, and analyzed by Matrix-assisted Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS). The peptide mass fingerprintings (PMF) was obtained. The proteins were identified by data searching in the Mascot database. Some differentially expressed proteins were confirmed by Western blotting. Results: The constructed two-dimensional gel electrophoresis profiles had high resolution and good reproducibility for both HS and IS groups. There were (754 + 64) differentially expressed protein spots (n = 5) in HS group and (777 ± 48) in LS group (n = 5). Some protein spots with differential ratio more than 2-fold were selected and analyzed by mass spectrometry. The average matching rate was 87.6% between the two groups. Eight differentially expressed proteins were successfully identified among which five proteins had overexpression and three proteins had weak expression in HS group. The results of Western blotting were consistant with proteomic screening. Conclusion: There was significant difference in protein profilings between HS and LS early cervical carcinoma after radiotherapy. The differentially expressed proteins may be radiosensitivity-associated proteins.
