G-CSF expression in human solid carcinomas and its effects on cell proliferation
- Author:
Dan SHA
1
Author Information
1. Cancer Center
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Gene expression;
Granulocyte colony-stimulating factor;
Tumor cells, cultured
- From:
Tumor
2007;27(10):799-802
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate G-CSF expression and rhG-CSF's effects on cell proliferation and its mechanism in human solid carcinoma cell lines. Methods: G-CSF expression was detected by human G-CSF ELISA Kit. Cell proliferation promoted by rhG-CSF was studied by cell count and MTT. The influence of rhG-CSF on KB cell cycle and apoptosis was analysed by flow cytometry. G-CSF receptor expression was detected by immunocytochemistry. Results: Cell line T-24 produced large amount of G-CSF; rhG-CSF promoted cell line KB proliferation in a dose-dependent manner and reached its maximal effect at a concentration of 100 ng/mL (P < 0.05); rhG-CSF antagonized G0/G1 phase block and reduced apoptosis rate of KB cells. Conclusion: Cell line T-24 expresses G-CSF; rhG-CSF promotes, KB cell proliferation by binding its G-CSF receptor, antagonizing G0/ G1 phase block and inhibiting apoptosis.
