Effect of hydroxycamptothecin on hypoxia-induced expression of HIF-1 α and VEGF genes in SiHa cells
- Author:
Wei SHI
1
Author Information
1. Cancer Center
- Publication Type:Journal Article
- Keywords:
Hydroxycamptothecin;
Hypoxia-inducible factor 1, alpha subunit;
Uterine cervical neoplasms;
Vascular endothelial growth factors
- From:
Tumor
2007;27(10):795-798
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effect of hydroxycamptothecin (HCPT) on hypoxia-induced expression of hypoxia inducible factor 1 alpha (HIF-1 α) and vascular endothelial growth factor (VEGF) genes in cervical cancer SiHa cells. Methods: Human cervical cancer SiHa cells were cultured under hypoxic conditions (37°C, 5% CO2, 1% O2) in vitro. The cultured cells were treated with different concentrations of HCPT for 24 h. The mRNA and protein expression of HIF-1α and VEGF were determined by semiquantitive RT-PCR and Western blot, respectively. The SiHa cells were also treated with HCPT under normal oxygen condition. SiHa cells in hypoxic control group were cultured under hypoxic conditions only. Results: There was no significant difference in the cytotoxicity induced by HCPT between hypoxic groups and normal control group. Under normal condition, SiHa cells did not express HIF-1α protein but weakly expressed VEGF protein. HCPT had no obvious effect on expression of HIF-1 α and VEGF genes. Hypoxia significantly induced up-regulation of the expression of HIF-1 α and VEGF. HCPT had significant inhibitory effects on the protein expression of HIF-1 α and mRNA and protein expression of VEGF induced by hypoxia in a dose-dependent manner but had no obvious influence on mRNA expression of HIF-1 α. Conclusion: HCPT inhibits hypoxia-induced over-expression of HIF-1 α protein and the downstream VEGF gene in SiHa cells.
