Inhibition of the proliferation and invasion of pancreatic cancer cells by MAT1 gene-targeted short interfering RNA in vitro
- Author:
Shi-Neng ZHANG
1
Author Information
1. Department of Gastroenterology
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Genes, MAT1;
Neoplasm invasiveness;
Pancreatic neoplasms;
RNA interference
- From:
Tumor
2007;27(10):783-786
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of silencing MAT1 gene by short interfering RNA (siRNA) on the proliferation and invasion of human pancreatic cancer cells and discuss the feasibility of using MAT1 gene as therapeutic target for treatment of pancreatic cancer. Methods: BxPC-3 cells were transfected with sequence-specific siRNA targeting MAT1 gene with liposome mediation. The mRNA and protein expression of MAT1 were confirmed by RT-PCR and Western blot, respectively. The cell proliferation was measured by Trypan blue exclusion staining. The invasion ability was determined by Boyden chamber model in vitro. Results: The mRNA and protein expression of MAT1 were significantly down-regulated by siRNA in BxPC-3 cells compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% at 24 h and 48 h, respectively (P <0.01). The cell proliferation and invasion ability of BxPC-3 cells were significantly inhibited in vitro (P < 0.01). Conclusion: The results suggest that siRNA targeting MAT1 gene inhibits the cell proliferation and invasion of BxPC-3 cells in vitro. MAT1 may be a potential target for gene therapy of human pancreatic cancer.
