Study on the feasibility of using magnetic iron oxide nanoparticles as wt-p 53 gene carrier for transfection of lung cancer cells
- Author:
Dong-Bo ZHOU
1
Author Information
1. Department of Respiratory Medicine
- Publication Type:Journal Article
- Keywords:
Genes, p 53;
Lung neoplasms;
Magnetic iron oxide nanoparticles
- From:
Tumor
2007;27(11):882-886
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To evaluate the feasibility of using magnetic iron oxide nanoparticles (pll-DCIONP) with poly-L-lysine modified surface as wild type p 53 gene carrier for transfection of lung cancer cells in vitro. Methods: The dextran-coated iron oxide nanoparticles (DCIONP) were synthesized with chemical precipitation method. The surface of DCIONP was modified by self-assembled poly-L-lysine to form particle complexes (pll-DCIONP). The configuration of pll-DCIONP was detected by scanning electron microscope. The diameter, size distribution and Zeta surface potential of pll-DCIONP was detected by Laser Particle Size Analyzer. The potential of adsorbing wt-p 53 gene and resisting DNase- I and blood serum digestion of pll-DCIONP/wt-p53 complex were analyzed by spectrophotometer and agarose gel electrophoresis, respectively. The pll-DCIONP was evaluated as a kind of wt-p53 gene carrier and transfected into human lung adenocarcinoma cell line A549/DDP in vitro. The mRNA and protein expression of intracellular p53 were tested by RT-PCR and Western blotting, respectively. Results: The diameter of the pll-DCIONP was between 60 and 80 nm. The Zeta surface potential of pll-DCIONP was + 19. 6 mV. At different pH conditions, the pll-DCIONP could adsorb wt-p 53 gene which was the strongest when the ratio of pII-DCIONP/wt-p53 was 1: 1 (m/m). The pll-DCIONP/wt-p 53 complex could resist DNase-I and blood serum digestion. The intracellular p 53 gene levels continuously increased in a time-dependent manner after transfection of human lung adenocarcinoma cells with pll-DCIONP/wt-p53 complex. The intracellular p 53 gene levels decreased in a time-dependent manner after transfection with the wt-p 53 DNA-lipofectamine complex. Conclusion: pll-DCIONP could be used as one of the ideal gene carriers for wt-p 53 gene delivery. It continuously and effectively mediates wt-p 53 gene transfection into lung cancer cells.
