Antitumor efficacy of photodynamic therapy-generated glioma vaccine from dendritic cells
- Author:
Shi-Xiang YUAN
1
Author Information
1. Department of Neurosurgery
- Publication Type:Journal Article
- Keywords:
Cancer vaccines;
Dendritic cells;
Photochemotherapy;
T-lymphocytes, cytotoxic
- From:
Tumor
2007;27(12):962-967
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the killing activity of cytotoxic T lymphocytes (CTLs) stimulated by dendritic cells (DCs) loaded with C 6 glioma antigen eluted by mild acid after photodynamic therapy(PDT) and test the direct influence of PDT on the immunogenicity of C6 glioma cells. Methods: Progenitors of DCs were isolated from rat bone marrow and cultured with rat recombinant interleukin-4 and rat recombinant granulocyte-macrophage colony stimulating factor to differentiate mature DC. After PDT, the surface antigen peptides were eluted by mild acid from the still adherent C 6 cells and the whole cell antigens were extracted from the supernatant. For the PDT-untreated C 6 cells, tumor antigens were made by freeze/thaw or mild acid elution method. Then the four types of antigens were used to pulse DCs to generate DC vaccines. SD rats were inoculated with the four DC vaccines to prepare antigen-specific CTLs. The killing activities of spleen CTLs were determined in vitro. Results: The mild acid-eluted surface antigens from PDT-treated C6 cells promoted the maturation of DCs most efficiently. CTLs stimulated by the DC vaccine had highest cell-killing activity in vitro (95.5 ± 1.6)%. The cell-killing rate was (90.2 ± 2.4) % for the CTLs pulsed by whole cell antigens from supernatant of PDT-treated C 6 glioma cells, (73.3 ± 2.7)% for the CTLs pulsed by acid-eluted antigen from PDF-untreated C 6 glioma cells, (63.6 ± 4.9)% for the CTLs pulsed by antigen from PDF-untreated and frozen-thawed C 6 glioma cells, and 0 for PBS control group. Conclusion: PDT directly enhancs immunogenicity of tumor cells. The DC vaccine generated by the antigen peptides eluted by mild acid from PDT-treated tumor cells has a wide prospect of application and research values.
