Effects of suppression of MTA1 expression by RNA interference on proliferation of human breast carcinoma cell line MDA-MB-231
- Author:
Ping ZHANG
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
MDA-MB-231 cells;
Metastasis associated gene;
RNA, small interfering
- From:
Tumor
2007;27(12):957-961
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the effects of suppressing MTA1 expression using RNA interference technique on the proliferation and cell cycle of human breast carcinoma cell line MDA-MB-231. Methods: We established three MDA-MB-231 cell lines which had stable expression of MTA1-targeted shRNA. The suppression of MTA1 expression was verified by RT-PCR and immunohistochemistry. Cell proliferation capacity was evaluated by cell counting and MTT assay. The cell cycle was analyzed by flow cytometry. Results: Expression of MTA1 mRNA was significantly down-regulated after silencing MTA 1 gene by RNA interference. RT-PCR results showed that the expression of MTA1 mRNA was down-regulated by 70.07%, 82.40%, 63.44% in MDA-MB-231 cells after stable transfection of three types of plasmids. The growth of MDA-MB-231 cells was inhibited significantly. Compared with the control group, the cell number significantly decreased. MTT assay and flow cytometry showed that cell proliferation capacity decreased and the cells were arrested at G0/G1 phase after silencing MTA 1 gene. Conclusion: Suppression of the expression of MTA1 in MDA-MB-231 cells effectively inhibited cell proliferation and induced cell cycle arrest. Silencing MTA 1 gene by RNA interference may be a useful approach of gene therapy for human breast carcinoma.
