Effects of inhibition of HER 2 expression by Newcastle disease virus on the proliferation of human gastric adenocarcinoma cell line BGC-823
- Author:
Kai-Yang LIU
1
Author Information
1. Experimental Center
- Publication Type:Journal Article
- Keywords:
Cell proliferation;
Gene expression;
Genes, erbB-2;
Newcastle disease virus;
Stomach neoplasms;
Tumor cells, cultured
- From:
Tumor
2007;27(12):944-947
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects of selective inhibition of HER 2 expression by Newcastle disease virus to the proliferation of human gastric adenocarcinoma cell line BGC-823. Methods: The expression of HER 2 mRNA and protein in Newcastle disease virus-infected gastric adenocarcinoma cells was determined by RT-PCR and Western blotting. The inhibitory effect of Newcastle disease virus on proliferation of BGC-823 cells were assessed by MTT assay. Apoptotic rate of BGC-823 cells was measured by flow cytometry (FCM). Results: The infection of BGC-823 cells with Newcastle disease virus successfully knocked down the expression of HER 2 at mRNA and protein levels. Down-regulation of HER 2 gene inhibited the proliferation and increased the apoptotic rate of BGC-823 cells compared with the control group (P < 0.01). Conclusions: Infection with Newcastle disease virus efficiently killed BGC-823 cells and induced apoptosis. The mechanism may be due to the inhibition of HER 2 gene expression in BGC-823 cells.
