The influence of β peptide dimmer modified by polyethylene glycol on anti-metastasis activity
- Author:
Jun ZHU
1
Author Information
1. Department of Pharmacy
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Cell adhesive molecules;
Dimmer of β peptide;
Neoplasm invasiveness;
Polyethylene glycols
- From:
Tumor
2007;27(12):931-934
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the influence of β peptide dimmer modified by PEG on anti-metastasis activity. Methods: Cell culture plates were coated with fibronectin (FN) as extracellular matrix (ECM). The influence of β 2 and β 2-PEG peptides on the adhesion of tumor cells to FN were observed. The effects of β 2 and β 2-PEG peptides on migration and invasion ability of tumor cells in reconstituted basement membrane were measured by using Transwell Boyden and Matrigel method. Results: Compared with negative control, β 2 and β 2-PEG peptide significantly inhibited the adhesion of SMMC-7721 and HCCLM 6 tumor cells to FN in a time- and dose-dependent manner (P < 0.05). The inhibitory effects of β 2-PEG were stronger than β 2 peptides (P < 0.05). The mobility and invasion of HCCLM 6 and SMMC-7721 cells were obviously inhibited by β 2 peptide and β 2-PEG (P < 0.05). For HCCLM 6 cells, β 2 peptide and β 2-PEG inhibited cell migration were 54.6% and 56.3% and suppressed cell invasion were 36. 8% and 46.6%, respectively. For SMMC-7721 cells, the migration inhibitory rate were 43.6% and 45.7% and invasion inhibitory rate was 33.6% and 35.9% by β 2 and β 2-PEG peptide, respectively. The difference were not significant before and after PEGylation. Conclusions: The β 2 and β 2-PEG peptides specifically inhibite the adhesion of tumor cells to FN in a time- and dose-dependent manner. The anti-adhesion effects are enhanced after PEG modification. The β 2 and β 2-PEG peptides obviously suppress migration and invasion of the two kinds of tumor cells. The difference is not significant before and after PEG modification.
