Isolation and identification of tumor-like stem cells from human osteosarcoma cell line
- Author:
Zhen OUYANG
1
Author Information
1. Institute of Orthopedics
- Publication Type:Journal Article
- Keywords:
Cell culture technique;
Cell line;
Osteosarcoma;
Tumor;
Tumor stem cells
- From:
Tumor
2008;28(1):36-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To isolate and identify tumor-like stem cells from human osteosarcoma cell line U2-OS. Methods: Tumor sphere cells were isolated from osteosarcoma cell line with serum-free culturing techniques. Flow cytometry was used for detecting the positive rates of Stro-1 protein in U2-OS cell line. The proliferation ability of sarcospheres was estimated by MTT assay. Stro-1+ cells were isolated using magnetic activated cell sorting system. RT-PCR was used to detect the transcription of Stro-1 mRNA in Stro-1+ cells. The Stro-1+ cells and Stro-1- cells were inoculated into NOD/SCID mice. Results: The results of flow cytometry showed that the percentage of Stro-1+ cells was 5.67% in U2-OS cells. Stro-1+ cells had capability of proliferation and differentiation. The over-expression of Stro-1 mRNA was observed in Stro-1+ cells. Inoculation of Stro-1+ cells induced osteosarcoma formation in NOD-SCID mice. Conclusion: Osteosarcoma-like stem cells exist in human osteosarcoma cell line U2-OS. They have self-renewal and multi-directional differentiation abilities.
