The expression of multi-drug resistance gene in breast cancer stem cells and its significance
- Author:
Zhi LI
1
Author Information
1. Department of Breast and Thyroid Surgery
- Publication Type:Journal Article
- Keywords:
BCRP;
Breast neoplasms;
Cell;
Gene;
MCF-7;
MDR 1;
Stem cells
- From:
Tumor
2008;28(2):129-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To detect the expression of multi-drug resistance genes MDR 1 and BCRP in breast cancer stem cells and differentiated cells as well as discuss their clinical significance. Methods: The breast cancer stem cells were separated from human primary breast cancer tissue and MCF-7 cells by flow cytometry-based selection on freshly isolated cells. The expressions of MDR 1 and BCRP in different subsets of cells was measured by real-time PCR. Results: Compared with breast cancer differentiated cells, the expressions of MDR 1 and BCRP in breast cancer stem cells were significantly higher (P < 0.01), and the proportion of stem cells markedly increased after chemotherapy (P < 0.01). Conclusion: Compared with breast cancer differentiated cells, breast cancer stem cells have stronger drug-resistance against chemotherapy via over-expression of multidrug resistance genes, which is one of the key factors for failure of chemotherapy and recurrence of breast cancer.
