Expressions of HIF-1α, GLUT1, MRP1, and GST-π in NSCLC tissues and their significance
10.3781/j.issn.1000-7431.2008.03.021
- Author:
Yong-Mei ZHANG
1
Author Information
1. Key Geriatric Laboratory
- Publication Type:Journal Article
- Keywords:
Cancer, non-small cell lung;
Glucose transporter type-1;
Glutathione-S-transferase;
Hypoxia-inducible factor-1, alpha subunit;
Multidrug resistance-associated proteins
- From:
Tumor
2008;28(3):263-267
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expressions of hypoxia-inducible factor-1α (HIF-1α), glucose transporter 1 (GLUT1), multidrug resistance associated protein (MRP1), and glutathione-S-transferases (GST-π) in non-small cell lung cancer (NSCLC) tissues and their relationship with the biological features of lung cancer. Methods: The expressions of HIF-1α, GLUT1, MRP1, and GST-π mRNA in 36 samples of NSCLC tissues and 12 samples of corresponding adjacent tissues were detected by RT-PCR and semiquantitative agarose gel electrophoresis. Their expression in various TNM staging cancer tissues was analyzed by SAS8.0 statistic software. Results: The expressions of the four genes significantly increased in NSCLC tissues than in the corresponding adjacent tissues (P < 0.01). The expressions of the four genes were significantly higher in the group with lymph node and distant metastasis than that in the groups without lymph node and distant metastasis (P < 0.01 or P < 0.05). The expressions of HIF-1α and GLUT1 mRNA were significantly increased in T3 and T4 staging cancer tissues than that in T1 and T2 staging cancer tissues (P < 0.01 or P < 0.05). The expressions of MRP1 and GST-π mRNA had no significant difference between NSCLC tissues at T1, T2, T3 and T4 staging(P >0.05). Conclusion: HIF-1α, GLUT1, MRP1, and GST-π were over-expressed in NSCLC tissues, which were associated with lymph node and distant metastasis. Expressions of HIF-1α and GLUT1 were elevated with the increase in the TNM staging but expressions of MRP1 and GST-π did not correlate with TNM staging. HIF-1α mRNA expression was positively related with the expression of GLUT1 mRNA (P < 0.01). The expression of HIF-1α mRNA was significantly related with the expression of MRP1 and GST-π mRNA in linear dependency (P <0.05). The expression of GLUT1 mRNA was not significantly related with the expression of MRP1 and GST-π mRNA (P > 0.05). The over-expressions of the four genes may be useful markers indicating the carcinogenesis, Progression, and deterioration of NSCLC.
