The relationship between NDRG-1 gene expression and the progression and metastasis of uterine cervical squamous cell carcinoma
10.3781/j.issn.1000-7431.2008.03.015
- Author:
Shao-Fei MA
1
Author Information
1. Department of Pathology
- Publication Type:Journal Article
- Keywords:
Gene expression;
Immunohistochemistry;
Reverse transcriptase polymerase chain reaction;
Uterine cervical neoplasms
- From:
Tumor
2008;28(3):238-241
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between N-myc downstream-regulated gene-1 (NDRG-1) gene expression and the progression and metastasis of uterine cervical squamous cell carcinoma (UCSCC). Methods: NDRG-1 protein expression was detected by immunohistochemistry (IHC) in 42 cases of UCSCC, 30 cases of high-grade squamous intraepithelial lesion (HSIL), 28 cases of low-grade squamous intraepithelial lesion (LSIL), and 20 cases of normal squamous epithelium. NDRG-1 mRNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in 10 cases of UCSCC and 10 cases of normal squamous epithelium. Results: The expressions of NDRG-1 mRNA and protein in UCSCC were significantly lower than normal squamous epithelium tissues (P < 0.01). From normal squamous epithelium to LSIL, HSIL and UCSCC, NDRG1 protein was gradually decreased during tumor carcinogenesis. The difference was significant (P < 0.01). NDRG-1 mRNA and protein in metastatic UCSCC were obviously lower than non metastatic UCSCC (P < 0.05). Conclusion: Down-regulation of NDRG1 mRNA and protein in UCSCC indicate that NDRG1 gene might correlate with carcinogenesis, progression, and metastasis of cervical squamous carcinoma.
