CCR1 and CCR5 mediated the effects of macrophage inflammatory protein-1α on the proliferation and migration of multiple myeloma cells
10.3781/j.issn.1000-7431.2008.03.013
- Author:
Xiao-Tao WANG
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- Keywords:
Cell movement;
Cell proliferation;
Macrophage inflammatory protein-1;
Multiple myeloma;
Receptors, chemokine
- From:
Tumor
2008;28(3):232-235
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the expression of chemokine receptors CCR1 and CCR5 in multiple myeloma (MM) cells and whether they mediated the effects of macrophage inflammatory protein 1 (MIF-1) alpha on the proliferation and migration of MM cells. Methods: CCR1 and CCR5 mRNA were quantified by reverse transcription polymerase chain reaction (RT-PCR) in MM cell lines KM3, SKO 007, and XG-6. Cell proliferation was detected by MIT assay and Transwell migration assay was performed to identify the cell migration. Results: KM3 and XG-6 MM cells expressed CCR1 and CCR5, but SKO007 expressed CCR5 only. Treatment with CCR1 and/or CCR5 antibodies reversed the effects of MIP-1α on proliferation and migration of the three cell lines compared with control (P < 0.05), but there was no significant difference among the three experimental groups (P > 0.05). Conclusion: KM3 and XG-6 MM cells expresss both CCR1 and CCR5 and SKO 007 expresss CCR5 only. Effects of MIP-1α on proliferation and migration were mediated by CCR1 or CCR5.
