Transcatheter hepatic artery infusion with recombinant human endostatin combined with TACE for rabbit VX2 hepatoma and evaluation on angiogenesis by CT perfusion
10.3781/j.issn.1000-7431.2008.03.008
- Author:
Deng-Ling ZHAO
1
Author Information
1. Clinical Medical College
- Publication Type:Journal Article
- Keywords:
Carcinoma, hepatocellular;
Chemoembolization, therapeutic;
Diagnostic imaging;
Neovasularization, pathologic
- From:
Tumor
2008;28(3):211-215
- CountryChina
- Language:Chinese
-
Abstract:
Objective: Rabbit VX2 hepatoma was treated with transcatheter hepatic artery infusion with recombinant human (rh) endostatin combined with transcatheter arterial chemoembolization (TACE). The therapeutic efficacy was evaluated and the peri-cancerous CT perfusion features as well as the expressions of microvascular density (MVD) and vascular endothelial growth factor (VEGF) were analyzed. Methods: Thirty rabbits with VX2 hepatoma were randomly divided into three groups with 10 rabbits in each group. Group A were treated with TACE and infused with rh-endostatin via hepatic artery. Group B were treated with TACE only. Group C were control group. Routine CT scanning was performed before therapy and CT perfusion imaging were performed at 2nd weeks after treatment to get the data of blood flow (BF), blood volume (BV) and capillary permeability surface (PS) area. The rabbits were sacrified immediately after scanning. The MVD and VEGF expressions in tumor tissue slides were detected by immunohistochemistry. Results: The hepatoma growth rate was significantly lower in group A and B than that in group C (P <0.01). The BF, BV, and PS were significantly increased and MVD and VEGF were significantly decreased in group A than those in group B and C (P < 0.05, P < 0.01). There was no significant difference in the value of BF, BV, PS, MVD, and VEGF between groups B and C (P > 0.05). The expression of MVD positively correlated with the expression of VEGF in linear dependency (P < 0.01). BF, BV, and PS had positive correlation with MVD and VEGF in group A (P < 0.05). BF, BV, and PS had no significant correlation with MVD and VEGF expression in group B (P > 0.05). Conclusion: The combination therapy of TACE and administration of recombinant human endostatin via hepatic artery infusion significantly slows down the growth speed of hepatoma and inhibits tumor angiogenesis. The parameters of CT perfusion imaging can not reflect the changes of pathological parameters such as MVD and VEGF at any therapeutic condition.
