Effect of breast cancer metastasis suppressor gene 1 on the migration of breast cancer cells
10.3781/j.issn.1000-7431.2008.03. 007
- Author:
Yin-Long YANG
1
Author Information
1. Department of Breast Surgery
- Publication Type:Journal Article
- Keywords:
Breast cancer metastasis suppressor gene 1;
Breast neoplasms;
Neoplasm metastasis
- From:
Tumor
2008;28(3):207-210
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the effect of breast cancer metastasis suppressor gene 1 (BRMS1) on the migration of human breast cancer cells. Methods: An eukaryotic expression vector containing BRMS1 was constructed and was transfected into human breast cancer MDA-MB-231-HM cells by using Lipofectin 2000. Expression of BRMS1 mRNA was detected by real-time PCR. The migration of the cells was assayed by Transwell test. Meanwhile, three pairs of siRNA targeting BRMS1 were designed. The most effective siRNA was screened by real-time PCR and transfected into MDA-MB-231 cells. The migration ability of the transfected cells was detected. Results: Compared with the cells transfected with empty vector, the number of MDA-MB-231-HM cells on the underlayer of transwell decreased by 51.9% after stably transfected with BRMS1. One pair of siRNA was selected which could notably down-regulated BRMS1 expression at mRNA level. After siRNA transfection, the number of MDA-MB-231 cells on the underlayer of transwell increased by 17.9%. Conclusion: BRMS1 suppresses the migration of breast cancer cells, which indicates that BRMS1 may suppress cancer distant metastasis by inhibiting migration of breast cancer cells.
