Expression of histone deacetylase 3 in acute leukemic cell lines
10.3781/j.issn.1000-7431.2008.04.010
- Author:
Hua ZHONG
1
Author Information
1. Department of Hematology
- Publication Type:Journal Article
- Keywords:
Acute;
Histone deacetylases;
Leukemia;
Myelocytic;
Tretinoin
- From:
Tumor
2008;28(4):313-316
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the expression of histone deacetylase 3 (HDAC3) in acute leukemic cell lines and to observe whether the HDAC3 expression is necessary for the transcriptional regulation by controlling proliferation and differentiation of leukemia cells. Methods: The HDAC3 mRNA and protein expression levels in 16 acute myeloid leukemic cell lines were assessed by real-time quantitative PCR (RTQ-PCR) and Western blotting, respectively. The mRNA and protein expression levels of HDAC3 were detected during differentiation of NB4 cells induced by all-trans retinoic acid (ATRA). Results: The mRNA expressions of HDAC3 were higher in NB4, NB4RA and NOMO-1 leukemic cell lines but the protein expression levels were not parallel with mRNA expressions. HDAC3 mRNA and protein expression had a decreased tendency during the ATRA-induced differentiation of NB4 cells. Conclusion: HDAC3 is necessary for proliferation of leukemic cells and the expression of HDAC3 in NB4 cell line is associated with cell differentiation and/or proliferation.
