Silencing PI3K by siRNA activated FoxO3a gene and inhibited the growth of xenografted breast carcinoma in nude mice
10.3781/j.issn.1000-7431.2008.04.009
- Author:
Wei-Yi HUANG
1
Author Information
1. Department of Oncology
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Mice;
Nude;
Phosphatidylinositol 3-kinase;
RNA interference;
Xenograft model antitumor assays
- From:
Tumor
2008;28(4):310-312
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of silencing PI3K by small interfering RNA (siRNA) on the expression of FOXO3a in human breast cancer MCF-7 cells and the growth of xenografted breast carcinoma in nude mice. Methods: Plasmids of siRNA for PI3K gene (Psilencer1.0-U6-PI3K) were constructed and xenografted tumor model was established in nude mice by subcutaneous inoculation of MCF-7 cells. Twenty-seven nude mice with xenografted tumor were divided randomly into 3 groups. Group 1 was intratumorally administered by Psilencer1.0-U6-PI3K plasmid. Group 2 and group 3 were intratumorally given Psilencer10-U6 plasmid and physiological saline (group 1), respectively. The tumor volume and survival time of nude mice were recorded. The expressions of PI3K and FOXO3a was measured by Western blotting. Results: The tumor size was significantly decreased and the average survival time was significantly enlongated in Psilencer1.0-U6-PI3K-treated group (P <0.01). Western blotting analysis showed that the protein expression of PI3K in xenografted tumor was markedly decreased and FOXO3a was significantly elevated compared with control groups (P <0.05). Conclusion: Psilencer1.0-U6-PI3K markedly silenced the expression of PI3K gene, up-regulated the expression of tumor suppressor gene FOXO3a, inhibited the growth of xenografted tumor, finally prolonged the survival time of nude mice bearing human breast carcinoma.
