The inhibitory effects of exogenous ING4 gene expression on the growth of breast cancer cells
10.3781/j.issn.1000-7431.2008.04.007
- Author:
Fang CAO
1
Author Information
1. Department of Biotechnology
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Breast neoplasms;
Gene;
Transfection;
Tumor suppressor
- From:
Tumor
2008;28(4):301-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To observe the inhibitory effects of ING4 gene expression on the growth of MCF-7 cells. Methods: ING4 mRNA expression in three breast cancer lines was detected by real-time fluorogentic quantitative-PCR (RFQ-PCR). pcDNA3.1(+) ING4 was transfected into MCF-7 cells. The proliferation activity of MCF-7 cells was measured by MTT assay and flow cytometry. Cell apoptotic ratios were analyzed by Annexin-V/PI double staining. Results: The expression of ING4 was markedly lower in MCF-7, MDA-MB-435, and MDA-MB-231 cells compared with normal breast tissues. Over expression of ING4 slowed down proliferation speed of MCF-7 cells, increased the cell proportion in G1 phase and decreased the proportion in S phase (P < 0.05). The apoptotic ratio of MCF-7 cells was significantly increased compared to control group (P < 0.05). RT-PCR revealed that expressions of p21 and bax gene were all up-regulated but p53 had no change. Conclusion: ING4 was associated with tumorigenesis of breast cancer. Over expression of ING4 inhibited the proliferation of human breast cancer MCF-7 cells.
